There are companies that provide services for antibody development. Companies like Bosterbio have a personal monoclonal antibody development platform that uses a single plasma cell assay technology that reveals the rabbit's immune repertoire. but to produce antibodies that work for your specific application.
The technical staff with decades of experience in the fields of microbiology, immunology, cell, biochemistry, and molecular biology will guide you through the critical process of developing the right antigen. The equally important next steps in developing a strategy to turn antigens into custom rabbit monoclonal antibodies that actually work and actually validate.
These companies provide detailed technical support in antigen design. Apart from that, they can also make antigens for you if needed. Whether it's a peptide or a recombinant proteid (from one of three expression systems including E. coli, baculovirus, or mammals), they can do it for you.
Alternatively, you can start with more than a pair of rabbits or have an immunization protocol for them to use. They'll answer most of your questions – just let them know!
Benefits of personal service for monoclonal antibodies:
Strong affinity: Due to the isolation of highly differentiated plasma cells, only antibodies with high affinity are selected.
Multiple Epitope Covers: The company takes advantage of the unique properties of the rabbit's immune system to generate antibodies against multiple epitopes for a single antigen.