Multiple immunoassays allow the detection of several different analytes in the same sample. A well-designed multiplex analysis can mean taking only one ampoule of blood from a patient, not 8 or more. Therefore the multiplex test can answer several questions at once.
Immunoassays use the specific affinity between antibody and antigen. Some immunological tests use an antigen as a probe to detect the binding of an antibody target, while other immunological tests use an antibody probe to detect an antigenic target. If you are looking for information on multiplex elisa then you can browse the internet.
Most immunoassays are single plexus, meaning that they can only be used to detect one antibody-antigen pair at a time; This is usually the case with most Enzyme Link ImmunoSorbent Assay (ELISA) tests. The idea of multiplex ELISA is interesting and usually demonstrates some kind of multiplex immunoassay based on antibody-antigen binding events.
Conventional ELISA is complex and requires a lot of binding and washing as well as enzyme systems that generate colourimetric or chemiluminescent labels to measure target concentrations in samples.
Enzymes used in ELISA
When ELISA is used to detect antigens, sandwich ELISA is the most common format. This format uses an antibody probe attached to a surface, most commonly a 96-well polystyrene plate. If the appropriate antigen is present in the sample, it will bind to the capture antibody.